Officers at North Yorkshire Police have the worst fitness levels of 32 forces surveyed in England and Wales, figures show.
The force had a 16.2% test failure rate, with Northamptonshire coming in second at 8.5% and Gloucestershire with 7.5%, the College of Policing said.
Overall, 352 officers failed in more than 13,000 tests that become compulsory next month.
Humberside and Surrey were the only forces that boasted 100% pass rates.
North Yorkshire's Assistant Chief Constable Paul Kennedy, said the study did not assess results of all tests officers undertook.
“The actual pass rate for North Yorkshire Police officers who have taken the fitness test is 94.6%, with 1,153 officers passing the test out of 1,219 who have taken it so far,” he explained.
“The results issued by the college today are taken from a small snapshot in time and include the results of only 74 tests.
“Support, advice and encouragement will be provided to any officers who are struggling to reach the required fitness level.”
Rose Bartlett, from the college, said results showed the vast majority of officers tested are fit.
The new tests were brought in after recommendations made by Chief Inspector of Constabulary Tom Winsor.
The endurance test involves a series of shuttle runs between two lines 50ft (15m) apart at a steadily increasing pace controlled by means of a speaker emitting “bleep” signals in decreasing time periods.
Officers will be expected to pass the bleep test on an annual basis.